Marcellus Wiley, a former NFL pro bowler, has come out in defence of women’s sport.

Wiley said he was not anti-transgender but was concerned about protecting girls and women, and that he wouldn’t be comfortable with males playing against his daughters.

"I have no issue with transgenders. I do have an issue with athletes who are transgendered trying to participate going from a transition of a man to a woman and now playing with the women," Wiley said. "And, I will say that. You all can try to Dave Chappelle me all you want. I am very clear on this. You can be a transgender. You can be the homie, but I’ll be damned if a male at birth turns into a female and tries to compete against my daughters. She ain’t out there. We out. 

"Don’t make this a human rights issue – this is a biological issue. Simple as that. Trust me, I am a man."

The former Buffalo Bills and San Diego Chargers star finished the video, saying it’s unfair for that to happen.

"I hate saying fair. It’s not even right. Forget fair, it’s not right."

Binary spokeswoman Kirralie Smith commended the male athlete.

“He is spot on, it is not right! Male and female divisions exist for a reason,” she said.

“If males can play as females then what is the point of separate divisions?

“Males have a biological advantage over girls and women that cannot be negated with costumes, name changes, drugs or genital mutilation surgery.”

Ms Smith said women deserve their own competitions to ensure safety, dignity, and fairness for everyone.

“Create a third category if transgender people refuse to play in their biological category,” she said.

“Protect women’s sport because it is the right thing to do.”